Hotspot

accD

LSC · 55931-57379 · 1449 bp

Back to Orchis

Pi 0.00069
MarkerSeek score 59.10
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1449
variable_sites
1
pi
0.00069
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 ATAGGGGGCATAAAGACTCT ACACCATCTTCTTCGTTTTGT 45.0 38.1 54.9 56.0 1579 bp 1.0 40.1
accD_p2 AATAGGGGGCATAAAGACTCT ACACCATCTTCTTCGTTTTGT 42.9 38.1 55.8 56.0 1580 bp 1.0 40.1
accD_p3 ATAGGGGGCATAAAGACTCT AACACCATCTTCTTCGTTTTGT 45.0 36.4 54.9 56.8 1580 bp 1.0 40.1
accD_p4 ATAGGGGGCATAAAGACTCT ACACCATCTTCTTCGTTTTGTT 45.0 36.4 54.9 56.8 1579 bp 1.0 40.1
accD_p5 AAATAGGGGGCATAAAGACTCT ACACCATCTTCTTCGTTTTGT 40.9 38.1 56.6 56.0 1581 bp 1.0 40.1