Hotspot

psaJ-rpl33

LSC · 64919-65477 · 559 bp

Back to Orchis

Pi 0
MarkerSeek score 59.10
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
559
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TTGGGTCTTTAGCGGGTCTA AGAAATGCCCGTCGATTCTT 50.0 45.0 58.1 57.6 749-756 bp 1.0 77.0
psaJ-rpl33_p2 GATGCCTTGTCATTCCCCTT AGAAATGCCCGTCGATTCTT 50.0 45.0 57.9 57.6 702-709 bp 1.0 76.4
psaJ-rpl33_p3 ATGGTTTGGGTCTTTAGCGG AGAAATGCCCGTCGATTCTT 50.0 45.0 58.2 57.6 754-761 bp 1.0 76.2
psaJ-rpl33_p4 TGGGTCTTTAGCGGGTCTAT AGAAATGCCCGTCGATTCTT 50.0 45.0 57.8 57.6 748-755 bp 1.0 76.2
psaJ-rpl33_p5 TGCCTTGTCATTCCCCTTTT AGAAATGCCCGTCGATTCTT 45.0 45.0 57.6 57.6 700-707 bp 1.0 74.4