Pi
0
MarkerSeek score
58.80
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1044-1045 bp | 1.0 | 79.0 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | CCGAGGCTCAATCCAATCAA | 55.0 | 50.0 | 57.9 | 58.0 | 1077-1078 bp | 1.0 | 78.7 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 1052-1053 bp | 1.0 | 78.5 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1076-1077 bp | 1.0 | 78.1 |
| trnT-UGU-trnL-UAA_p5 | GGAATCGAACCGATGACCAT | CCGAGGCTCAATCCAATCAA | 50.0 | 50.0 | 57.8 | 58.0 | 1109-1110 bp | 1.0 | 77.8 |