Hotspot

trnT-GGU-psbD

LSC · 30848-31856 · 1009 bp

Back to Orchis

Pi 0.000991
MarkerSeek score 59.30
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1009
variable_sites
1
pi
0.000991
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GACCAGCCTACAAACACGAA 55.0 50.0 58.1 58.1 1168 bp 1.0 78.5
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GACCAGCCTACAAACACGAA 50.0 50.0 58.1 58.1 1171 bp 1.0 78.4
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG CCCTCCGTAACCAATCATCC 55.0 55.0 58.1 57.7 1143 bp 1.0 77.5
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA CCCTCCGTAACCAATCATCC 50.0 55.0 58.1 57.7 1146 bp 1.0 77.3
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG CAGCCTACAAACACGAAACG 55.0 50.0 58.1 57.7 1165 bp 1.0 77.2