Hotspot

rpoB-trnC-GCA

LSC · 25643-26857 · 1215 bp

Back to Orchis

Pi 0.00165
MarkerSeek score 59.70
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1215
variable_sites
2
pi
0.00165
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AATTCTTCCGTCAAGCCCTG TAAAGGATTTGCAGTCCCCC 50.0 50.0 58.2 57.5 1363 bp 1.0 74.5
rpoB-trnC-GCA_p2 AATTCTTCCGTCAAGCCCTG CGACACCCAGATTCGAACTG 50.0 55.0 58.2 58.6 1387 bp 1.0 73.5
rpoB-trnC-GCA_p3 CCGGGTATTGTGGACATTCC TAAAGGATTTGCAGTCCCCC 55.0 50.0 58.3 57.5 1300 bp 1.0 73.3
rpoB-trnC-GCA_p4 CGGGTATTGTGGACATTCCC TAAAGGATTTGCAGTCCCCC 55.0 50.0 58.3 57.5 1299 bp 1.0 73.3
rpoB-trnC-GCA_p5 AATTCTTCCGTCAAGCCCTG TTTGTTGATCAGGCGACACC 50.0 50.0 58.2 58.8 1400 bp 1.0 72.6