Hotspot

matK-trnK-UUU

LSC · 3123-4166 · 1044 bp

Back to Orchis

Pi 0.00192
MarkerSeek score 59.90
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
1044
variable_sites
2
pi
0.00192
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TCCTGAAGGAGTAGCGGATA ATCCGGGTTGCTAACTCAAC 50.0 50.0 57.0 57.9 1168-1169 bp 1.0 70.9
matK-trnK-UUU_p2 TCCTGAAGGAGTAGCGGATA AACGGTAGAGTACTCGGCTT 50.0 50.0 57.0 58.2 1151-1152 bp 1.0 70.4
matK-trnK-UUU_p3 TCCTGAAGGAGTAGCGGATA ACGGTAGAGTACTCGGCTTT 50.0 50.0 57.0 58.2 1150-1151 bp 1.0 70.4
matK-trnK-UUU_p4 TCCTGAAGGAGTAGCGGATA GATCCGGGTTGCTAACTCAA 50.0 50.0 57.0 57.6 1169-1170 bp 1.0 68.6
matK-trnK-UUU_p5 TCCTGAAGGAGTAGCGGATA TTGATCCGGGTTGCTAACTC 50.0 50.0 57.0 57.6 1171-1172 bp 1.0 68.6