Hotspot

ycf1

IRb · 111738-117308 · 5571 bp

Back to Orbea

Pi 0.0147
MarkerSeek score 46.60
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
IRb
length_bp
5571
variable_sites
81
pi
0.0147
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCCCAGACTCAATATACAATTTCT TGTTACTAACTTGGGTTTGTGA 33.3 36.4 55.3 55.5 227-260 bp 1.0 46.6
ycf1_p2 TCCCAGACTCAATATACAATTTCT TTGTTACTAACTTGGGTTTGTGA 33.3 34.8 55.3 56.2 228-261 bp 1.0 46.6
ycf1_p3 TCCCAGACTCAATATACAATTTCTT TGTTACTAACTTGGGTTTGTGA 32.0 36.4 56.0 55.5 227-260 bp 1.0 46.6
ycf1_p4 TTCCCAGACTCAATATACAATTTCT TGTTACTAACTTGGGTTTGTGA 32.0 36.4 56.0 55.5 228-261 bp 1.0 46.6
ycf1_p5 TCCCAGACTCAATATACAATTTC TGTTACTAACTTGGGTTTGTGA 34.8 36.4 53.9 55.5 227-260 bp 1.0 46.6