Hotspot

trnfM-CAU

LSC · 38108-38181 · 74 bp

Back to Orbea

Pi 0.0405
MarkerSeek score 75.80
Primer available yes
Label trnfM-CAU

Feature Metadata

label_name
trnfM-CAU
feature_type
tRNA
region
LSC
length_bp
74
variable_sites
3
pi
0.0405
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU_p1 TCTCTTTGCCAGGGAGAAGA TTCATCGACGTTGTTTTGCG 50.0 45.0 58.0 58.0 609-722 bp 1.0 84.5
trnfM-CAU_p2 GTGCCCGTTGTATTTGCTTC TTCATCGACGTTGTTTTGCG 50.0 45.0 58.0 58.0 1098-1194 bp 1.0 84.4
trnfM-CAU_p3 ATTAGCGTGCCCGTTGTATT TTCATCGACGTTGTTTTGCG 45.0 45.0 58.0 58.0 1104-1200 bp 1.0 84.3
trnfM-CAU_p4 GTGCCCGTTGTATTTGCTTC GCAAAGTTCCGTCATTGAGC 50.0 50.0 58.0 58.0 1179-1275 bp 1.0 84.2
trnfM-CAU_p5 GTGCCCGTTGTATTTGCTTC CAATCCCCACCACGGAATAG 50.0 55.0 58.0 58.0 1132-1228 bp 1.0 84.2