Hotspot

rps2

LSC · 15923-16633 · 711 bp

Pi 0.00563

MarkerSeek score 62.00

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 4
pi: 0.00563
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	CAAGGTCTGCCCAGTTATTT	GCCAATCTACGGTAGAGCAG	45.0	55.0	55.6	57.9	927-948 bp	1.0	62.0
rps2_p2	CAAGGTCTGCCCAGTTATTT	AGCCAATCTACGGTAGAGCA	45.0	50.0	55.6	58.2	928-949 bp	1.0	61.4
rps2_p3	CAAGGTCTGCCCAGTTATTTT	GCCAATCTACGGTAGAGCAG	42.9	55.0	56.4	57.9	927-948 bp	1.0	60.3
rps2_p4	CAAGGTCTGCCCAGTTATTTT	AGCCAATCTACGGTAGAGCA	42.9	50.0	56.4	58.2	928-949 bp	1.0	59.7
rps2_p5	CAAGGTCTGCCCAGTTATTTTT	GCCAATCTACGGTAGAGCAG	40.9	55.0	57.1	57.9	927-948 bp	1.0	58.1