Hotspot

accD

LSC · 59532-61085 · 1554 bp

Back to Orbea

Pi 0.0171
MarkerSeek score 61.80
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1554
variable_sites
25
pi
0.0171
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 TCCAATAACTATTCATCTATGGT AGTTCACCAAACAAAATGCCT 30.4 38.1 52.4 56.8 1678-1823 bp 1.0 45.1
accD_p2 ATCCAATAACTATTCATCTATGGT AGTTCACCAAACAAAATGCCT 29.2 38.1 53.1 56.8 1679-1824 bp 1.0 45.1
accD_p3 TCCAATAACTATTCATCTATGGT AGTTCACCAAACAAAATGCC 30.4 40.0 52.4 55.2 1678-1823 bp 1.0 45.1
accD_p4 TCCAATAACTATTCATCTATGGT GTTCACCAAACAAAATGCCT 30.4 40.0 52.4 55.2 1677-1822 bp 1.0 45.1
accD_p5 AATCCAATAACTATTCATCTATGGT AGTTCACCAAACAAAATGCCT 28.0 38.1 53.9 56.8 1680-1825 bp 1.0 45.1