Hotspot

ycf1-trnN-GUU

IRa · 136783-137814 · 1032 bp

Back to Orbea

Pi 0.027
MarkerSeek score 49.30
Primer available yes
Label ycf1-trnN-GUU

Feature Metadata

label_name
ycf1-trnN-GUU
feature_type
igs
region
IRa
length_bp
1032
variable_sites
8
pi
0.027
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.29
markerseek_score
49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-trnN-GUU_p1 AATGGGTTCCGGTATTCTGC CAATTGTGACCTCTCGGGAG 50.0 55.0 57.9 58.0 1230-1939 bp 1.0 84.7
ycf1-trnN-GUU_p2 AATGGGTTCCGGTATTCTGC AGAATGATGGGGATAGCGGA 50.0 50.0 57.9 58.0 1502-2211 bp 1.0 84.5
ycf1-trnN-GUU_p3 AATGGGTTCCGGTATTCTGC AGATAAACGCGGCTTCACAT 50.0 45.0 57.9 58.0 1728-2437 bp 1.0 84.4
ycf1-trnN-GUU_p4 AATGGGTTCCGGTATTCTGC ATAAACGCGGCTTCACATCT 50.0 45.0 57.9 58.0 1726-2435 bp 1.0 84.4
ycf1-trnN-GUU_p5 AATGGGTTCCGGTATTCTGC TTGTGACCTCTCGGGAGAAT 50.0 50.0 57.9 58.1 1227-1936 bp 1.0 84.4