Hotspot

rps12-clpP1

LSC · 72050-72160 · 111 bp

Back to Orbea

Pi 0.0901
MarkerSeek score 71.60
Primer available yes
Label rps12-clpP1

Feature Metadata

label_name
rps12-clpP1
feature_type
igs
region
LSC
length_bp
111
variable_sites
10
pi
0.0901
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-clpP1_p1 AATTGAGATTTGCGCCAACG CATTTAATCCCCAGGGTCCG 45.0 55.0 58.0 58.0 891-925 bp 1.0 88.2
rps12-clpP1_p2 AATTGAGATTTGCGCCAACG ATGTCAGCAACAGAAGCACA 45.0 45.0 58.0 58.0 476-502 bp 1.0 82.4
rps12-clpP1_p3 AATTGAGATTTGCGCCAACG CTCGCACGGGTTTATACACA 45.0 50.0 58.0 58.0 551 bp 0.5 59.8
rps12-clpP1_p4 TGCTACCAATGGAGAATCGC CTCGCACGGGTTTATACACA 50.0 50.0 58.0 58.0 582 bp 0.5 59.6
rps12-clpP1_p5 TAGCTGACCCTCTTAGTCCG CTCGCACGGGTTTATACACA 55.0 50.0 57.9 58.0 802 bp 0.5 59.6