Hotspot

psbE-petL

LSC · 66599-67733 · 1135 bp

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Pi 0.00712
MarkerSeek score 63.80
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1135
variable_sites
8
pi
0.00712
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 TGACAAATAACCAACCCGCA AAGCCGCTAGTAGAAAACCA 45.0 45.0 58.0 56.9 1282-1293 bp 1.0 71.9
psbE-petL_p2 TGACAAATAACCAACCCGCA AGCCGCTAGTAGAAAACCAA 45.0 45.0 58.0 56.9 1281-1292 bp 1.0 71.9
psbE-petL_p3 CAACCCGCAATGAATAGGGA AAGCCGCTAGTAGAAAACCA 50.0 45.0 57.9 56.9 1271-1282 bp 1.0 71.6
psbE-petL_p4 CAACCCGCAATGAATAGGGA AGCCGCTAGTAGAAAACCAA 50.0 45.0 57.9 56.9 1270-1281 bp 1.0 71.6
psbE-petL_p5 AGAACGTTCTCCTGTGCTTC AGCCGCTAGTAGAAAACCAA 50.0 45.0 57.8 56.9 1193-1204 bp 1.0 70.9