Hotspot

atpB-rbcL

LSC · 56843-57634 · 792 bp

Back to Orbea

Pi 0
MarkerSeek score 62.20
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
792
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CTACATCTAGTACCGGGCCA AACACCGGCTTTGAATCCAA 55.0 45.0 58.0 58.3 915-943 bp 1.0 79.9
atpB-rbcL_p2 GCGCAAAGGCTACATCTAGT AACACCGGCTTTGAATCCAA 50.0 45.0 58.1 58.3 924-952 bp 1.0 79.5
atpB-rbcL_p3 GGAAACCCCAGAACCAGAAG AACACCGGCTTTGAATCCAA 55.0 45.0 58.1 58.3 854-882 bp 1.0 79.5
atpB-rbcL_p4 CGATACGTCCCAGGTTGTTT AACACCGGCTTTGAATCCAA 50.0 45.0 57.9 58.3 885-913 bp 1.0 79.4
atpB-rbcL_p5 TCTAGTACCGGGCCAATGAT AACACCGGCTTTGAATCCAA 50.0 45.0 57.9 58.3 910-938 bp 1.0 79.2