Hotspot

trnK-UUU-rps16

LSC · 4332-5131 · 800 bp

Back to Orbea

Pi 0.00769
MarkerSeek score 63.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
800
variable_sites
6
pi
0.00769
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TCAAAAGGGTGGGGGTTTTT 55.0 45.0 57.9 57.7 951-953 bp 1.0 79.4
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC TTCAAAAGGGTGGGGGTTTT 55.0 45.0 57.9 57.7 952-954 bp 1.0 79.4
trnK-UUU-rps16_p3 CCGATGGTATGGACGAATCC TTTCAAAAGGGTGGGGGTTT 55.0 45.0 57.9 57.7 953-955 bp 1.0 79.4
trnK-UUU-rps16_p4 CCGATGGTATGGACGAATCC TTTTCAAAAGGGTGGGGGTT 55.0 45.0 57.9 57.7 954-956 bp 1.0 79.4
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TCAAAAGGGTGGGGGTTTTT 50.0 45.0 58.2 57.7 889-891 bp 1.0 78.8