Hotspot

ycf2

Genome · 5833-12406 · 6574 bp

Back to Opuntia

Pi 0.00953

MarkerSeek score 33.60

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 6574
variable_sites: 103
pi: 0.00953
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.05
markerseek_score: 33.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	AATCATGGTCCTTGCGGATC	CTTTGACCCCCGAATTTGGA	50.0	50.0	58.0	58.1	512 bp	0.5	59.9
ycf2_p2	AATCATGGTCCTTGCGGATC	TCTTTGACCCCCGAATTTGG	50.0	50.0	58.0	58.1	513 bp	0.5	59.9
ycf2_p3	AATCATGGTCCTTGCGGATC	TTTGACCCCCGAATTTGGAG	50.0	50.0	58.0	58.1	511 bp	0.5	59.9
ycf2_p4	GTACGGATTCTTCCCCCAAC	CTTTGACCCCCGAATTTGGA	55.0	50.0	58.0	58.1	817 bp	0.5	59.8
ycf2_p5	GTACGGATTCTTCCCCCAAC	TTTGACCCCCGAATTTGGAG	55.0	50.0	58.0	58.1	816 bp	0.5	59.8