Hotspot

rpl32-ndhF

Genome · 136270-137690 · 1421 bp

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Pi 0.00246

MarkerSeek score 60.80

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: Genome
length_bp: 1421
variable_sites: 7
pi: 0.00246
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TTAACGCTGCCCAATATCCC	GAACCGGAAGTGGAGTGAAG	50.0	55.0	58.0	58.2	1331-1632 bp	1.0	82.6
rpl32-ndhF_p2	TTAACGCTGCCCAATATCCC	AACAAAGGAACCGGAAGTGG	50.0	50.0	58.0	58.3	1338-1639 bp	1.0	81.7
rpl32-ndhF_p3	TTAACGCTGCCCAATATCCC	TGTTGTCGTCGGAAAAAGGA	50.0	45.0	58.0	57.7	1375-1676 bp	1.0	81.7
rpl32-ndhF_p4	AAAAGCTTTTAACGCTGCCC	GAACCGGAAGTGGAGTGAAG	45.0	55.0	57.9	58.2	1339-1640 bp	1.0	81.6
rpl32-ndhF_p5	AAAAGCTTTTAACGCTGCCC	AACAAAGGAACCGGAAGTGG	45.0	50.0	57.9	58.3	1346-1647 bp	1.0	80.7