Hotspot

ycf1-rpl32

Genome · 135942-135999 · 58 bp

Back to Opuntia

Pi 0.0304

MarkerSeek score 56.10

Primer available yes

Label ycf1-rpl32

Feature Metadata

label_name: ycf1-rpl32
feature_type: igs
region: Genome
length_bp: 58
variable_sites: 4
pi: 0.0304
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rpl32_p1	AGCTAGAGATGCGGAATTGC	GGGATATTGGGCAGCGTTAA	50.0	50.0	58.1	58.0	301-305 bp	1.0	82.3
ycf1-rpl32_p2	ACCAGTTGATACGGCTACCT	GGGATATTGGGCAGCGTTAA	50.0	50.0	58.1	58.0	463-467 bp	1.0	81.3
ycf1-rpl32_p3	AGCTAGAGATGCGGAATTGC	ACGAACAATAGAAGTCGGCC	50.0	50.0	58.1	58.0	180-185 bp	0.5	63.8
ycf1-rpl32_p4	ACCAGTTGATACGGCTACCT	ACGAACAATAGAAGTCGGCC	50.0	50.0	58.1	58.0	342-347 bp	0.5	61.9
ycf1-rpl32_p5	GCTACCTCCTTCTCGATTGC	ACGAACAATAGAAGTCGGCC	55.0	50.0	58.1	58.0	329-334 bp	0.5	61.7