Hotspot

trnF-GAA-ndhJ

Genome · 68228-69013 · 786 bp

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Pi 0.0165

MarkerSeek score 63.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: Genome
length_bp: 786
variable_sites: 32
pi: 0.0165
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	928-978 bp	1.0	82.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	892-942 bp	1.0	82.3
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	TAATCACCCACGCCTGAAAC	55.0	50.0	58.0	58.2	965-1015 bp	1.0	81.7
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	916-966 bp	1.0	81.6
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TAATCACCCACGCCTGAAAC	50.0	50.0	58.1	58.2	929-979 bp	1.0	81.4