Hotspot

accD

LSC · 59389-60918 · 1530 bp

Back to Oplopanax

Pi 0.000654

MarkerSeek score 53.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1530
variable_sites: 1
pi: 0.000654
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCCTGGTGATTGGATTTATATGT	CAACAAAGAAACCCCGTTCG	34.8	50.0	55.1	57.9	1682 bp	1.0	40.0
accD_p2	TCCTGGTGATTGGATTTATATGT	TGTCAACAAAGAAACCCCGT	34.8	45.0	55.1	57.9	1685 bp	1.0	40.0
accD_p3	AGTTAATAATCCTGGTGATTGGA	CAACAAAGAAACCCCGTTCG	34.8	50.0	55.1	57.9	1691 bp	1.0	40.0
accD_p4	AGTTAATAATCCTGGTGATTGGA	TGTCAACAAAGAAACCCCGT	34.8	45.0	55.1	57.9	1694 bp	1.0	40.0
accD_p5	TCCTGGTGATTGGATTTATATGT	AACAAAGAAACCCCGTTCGT	34.8	45.0	55.1	58.2	1681 bp	1.0	40.0