Hotspot

ndhF-rpl32

SSC · 114250-115433 · 1184 bp

Back to Oplopanax

Pi 0.00338

MarkerSeek score 60.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1184
variable_sites: 4
pi: 0.00338
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	TAAGGCTGCCCAATATCCCT	50.0	50.0	58.0	58.2	1369 bp	1.0	78.7
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1367 bp	1.0	78.6
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1368 bp	1.0	78.6
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	GACAACGCTTTTAAGGCTGC	50.0	50.0	58.0	58.3	1380 bp	1.0	77.5
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	GACAACGCTTTTAAGGCTGC	45.0	50.0	58.0	58.3	1378 bp	1.0	77.4