Hotspot

trnN-GUU-ndhF

IRb · 110215-112023 · 1809 bp

Back to Oplopanax

Pi 0.00221

MarkerSeek score 47.20

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1809
variable_sites: 4
pi: 0.00221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	ATCTCCCCAGGTAGGATTCG	GTTGGTCGTGTAATCGTGGT	55.0	50.0	58.0	58.2	2140-2143 bp	1.0	78.3
trnN-GUU-ndhF_p2	ATCTCCCCAGGTAGGATTCG	TGGTTGGTCGTGTAATCGTG	55.0	50.0	58.0	58.2	2142-2145 bp	1.0	78.3
trnN-GUU-ndhF_p3	GGCAGAATAGCAGAGCAAGT	GTTGGTCGTGTAATCGTGGT	50.0	50.0	58.0	58.2	2506-2509 bp	1.0	78.2
trnN-GUU-ndhF_p4	GGCAGAATAGCAGAGCAAGT	TGGTTGGTCGTGTAATCGTG	50.0	50.0	58.0	58.2	2508-2511 bp	1.0	78.2
trnN-GUU-ndhF_p5	GAACTTGGGTGTGGGTCTTT	GTTGGTCGTGTAATCGTGGT	50.0	50.0	57.9	58.2	2649-2652 bp	1.0	77.9