Hotspot

rbcL-accD

LSC · 58716-59388 · 673 bp

Back to Oplopanax

Pi 0.00446

MarkerSeek score 61.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 673
variable_sites: 3
pi: 0.00446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	CACACCTGTGTTCGAACTCT	50.0	50.0	57.9	57.8	842 bp	1.0	77.6
rbcL-accD_p2	TGCTAAATGGAGCCCTGAAC	CACACCTGTGTTCGAACTCT	50.0	50.0	57.9	57.8	867 bp	1.0	77.4
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	TACTTAGCCCACACCTGTGT	50.0	50.0	57.9	58.3	851 bp	1.0	77.2
rbcL-accD_p4	TGCTAAATGGAGCCCTGAAC	TACTTAGCCCACACCTGTGT	50.0	50.0	57.9	58.3	876 bp	1.0	77.0
rbcL-accD_p5	GCTGCTTGTGAGGTATGGAA	CTTAGCCCACACCTGTGTTC	50.0	55.0	57.9	58.5	849 bp	1.0	75.6