Hotspot

psaA-ycf3

LSC · 43831-44549 · 719 bp

Back to Oplopanax

Pi 0.00139

MarkerSeek score 59.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 719
variable_sites: 1
pi: 0.00139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	897-904 bp	1.0	78.8
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TATGGCGCTTACTCCTGGTA	50.0	50.0	57.9	57.9	869-876 bp	1.0	78.6
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	899-906 bp	1.0	78.0
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	826-833 bp	1.0	77.9
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	830-837 bp	1.0	77.6