Hotspot

ndhF

SSC · 107903-110128 · 2226 bp

Back to Opisthopappus

Pi 0.0018

MarkerSeek score 45.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 4
pi: 0.0018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTAGCACTGGGTAGACCTCA	AGCTAAGATCCGGTGAATCG	55.0	50.0	57.9	57.2	2636-2646 bp	1.0	72.9
ndhF_p2	TCGTTGTGGTCGGACTCTAT	AGCTAAGATCCGGTGAATCG	50.0	50.0	58.2	57.2	2815-2825 bp	1.0	72.6
ndhF_p3	ATCAATTCGGTCGTTGTGGT	AGCTAAGATCCGGTGAATCG	45.0	50.0	57.8	57.2	2825-2835 bp	1.0	72.6
ndhF_p4	CTAGCACTGGGTAGACCTCA	CGAAAGAGCTAAGATCCGGT	55.0	50.0	57.9	57.1	2642-2652 bp	1.0	72.3
ndhF_p5	TCGTTGTGGTCGGACTCTAT	CGAAAGAGCTAAGATCCGGT	50.0	50.0	58.2	57.1	2821-2831 bp	1.0	72.1