Hotspot

accD

LSC · 56853-58304 · 1452 bp

Back to Opisthopappus

Pi 0.00138

MarkerSeek score 54.00

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1452
variable_sites: 2
pi: 0.00138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TGCAAATATGGAGCAAGAAAACT	TCCAGCAGGGATTCTTAGTCA	34.8	47.6	57.3	58.2	1589 bp	1.0	40.9
accD_p2	TGCAAATATGGAGCAAGAAAACT	CCAGCAGGGATTCTTAGTCA	34.8	50.0	57.3	56.6	1588 bp	1.0	40.1
accD_p3	GCAAATATGGAGCAAGAAAACTCT	TCCAGCAGGGATTCTTAGTCA	37.5	47.6	58.0	58.2	1588 bp	1.0	40.1
accD_p4	TGCAAATATGGAGCAAGAAAAC	TCCAGCAGGGATTCTTAGTCA	36.4	47.6	55.9	58.2	1589 bp	1.0	40.1
accD_p5	TGCAAATATGGAGCAAGAAAACT	CCAGCAGGGATTCTTAGTCAA	34.8	47.6	57.3	57.4	1588 bp	1.0	40.1