Hotspot

rpl32-trnL-UAG

SSC · 111301-112170 · 870 bp

Back to Opisthopappus

Pi 0.00346

MarkerSeek score 61.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 870
variable_sites: 3
pi: 0.00346
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCGGCATTAAAAGCTTTGT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	987-997 bp	1.0	79.8
rpl32-trnL-UAG_p2	GGCGGCATTAAAAGCTTTGT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	988-998 bp	1.0	79.8
rpl32-trnL-UAG_p3	AAAGGATATTGGGCGGCATT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	998-1008 bp	1.0	79.7
rpl32-trnL-UAG_p4	AAAGGATATTGGGCGGCATT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	999-1009 bp	1.0	79.7
rpl32-trnL-UAG_p5	GGCGGCATTAAAAGCTTTGT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	997-1007 bp	1.0	78.9