Hotspot

ndhF-rpl32

SSC · 110129-111135 · 1007 bp

Back to Opisthopappus

Pi 0.00303
MarkerSeek score 62.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1007
variable_sites
3
pi
0.00303
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTTTTTGTTGCCGTCGGAAA ACAAAGCTTTTAATGCCGCC 45.0 45.0 57.8 57.9 1196-1208 bp 1.0 79.1
ndhF-rpl32_p2 CTTTTTGTTGCCGTCGGAAA AATGCCGCCCAATATCCTTT 45.0 45.0 57.8 57.9 1185-1197 bp 1.0 79.0
ndhF-rpl32_p3 AAGACTTTTTGTTGCCGTCG ACAAAGCTTTTAATGCCGCC 45.0 45.0 57.5 57.9 1200-1212 bp 1.0 76.9
ndhF-rpl32_p4 ACGAAGACTTTTTGTTGCCG ACAAAGCTTTTAATGCCGCC 45.0 45.0 57.5 57.9 1203-1215 bp 1.0 76.9
ndhF-rpl32_p5 CGAAGACTTTTTGTTGCCGT ACAAAGCTTTTAATGCCGCC 45.0 45.0 57.5 57.9 1202-1214 bp 1.0 76.9