Hotspot

rpl16-rps3

LSC · 80299-81480 · 1182 bp

Back to Opisthopappus

Pi 0.000862
MarkerSeek score 61.00
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
1182
variable_sites
1
pi
0.000862
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 CAAGTGCCTGAAGAGCGTAT GAGGGTAGGGTTCCACTACA 50.0 55.0 58.0 57.8 1392-1414 bp 1.0 79.2
rpl16-rps3_p2 CAAGTGCCTGAAGAGCGTAT AGAGGGTAGGGTTCCACTAC 50.0 55.0 58.0 57.5 1393-1415 bp 1.0 76.8
rpl16-rps3_p3 GGGTTCAAGTGCCTGAAGAG GAGGGTAGGGTTCCACTACA 55.0 55.0 58.5 57.8 1397-1419 bp 1.0 75.6
rpl16-rps3_p4 CAAGTGCCTGAAGAGCGTAT AGGGTAGGGTTCCACTACAA 50.0 50.0 58.0 57.3 1391-1413 bp 1.0 75.4
rpl16-rps3_p5 CAAGTGCCTGAAGAGCGTAT GAGAGGGTAGGGTTCCACTA 50.0 55.0 58.0 57.2 1394-1416 bp 1.0 74.4