Hotspot

trnP-UGG-psaJ

LSC · 65591-65898 · 308 bp

Back to Opisthopappus

Pi 0.00649
MarkerSeek score 47.50
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
308
variable_sites
2
pi
0.00649
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 398 bp 1.0 79.3
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 397 bp 1.0 79.3
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 409 bp 1.0 79.2
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 408 bp 1.0 79.2
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 411 bp 1.0 79.2