Hotspot

petA-psbJ

LSC · 61975-62713 · 739 bp

Back to Opisthopappus

Pi 0.00136

MarkerSeek score 60.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 739
variable_sites: 1
pi: 0.00136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGCATCTGTGATTTTGGCAC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.4	57.6	845-850 bp	1.0	74.6
petA-psbJ_p2	TCGCATCTGTGATTTTGGCA	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.5	57.6	846-851 bp	1.0	73.9
petA-psbJ_p3	CGCATCTGTGATTTTGGCAC	TGTGATCGGTTTAGTAGGCG	50.0	50.0	58.4	57.4	883-888 bp	1.0	72.9
petA-psbJ_p4	CGCATCTGTGATTTTGGCAC	GTGATCGGTTTAGTAGGCGT	50.0	50.0	58.4	57.4	882-887 bp	1.0	72.9
petA-psbJ_p5	TTCGCATCTGTGATTTTGGC	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.4	57.6	847-852 bp	1.0	72.6