Hotspot

trnF-GAA-ndhJ

LSC · 47606-48304 · 699 bp

Back to Opisthopappus

Pi 0.00429

MarkerSeek score 61.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 3
pi: 0.00429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	802-811 bp	1.0	70.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	801-810 bp	1.0	70.1
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	50.0	47.6	58.1	57.7	809-818 bp	1.0	69.5
trnF-GAA-ndhJ_p4	AATGAGGATGCGACATCAGG	TGGATAGGATGGCCTTTACG	50.0	50.0	57.7	56.8	865-874 bp	1.0	68.5
trnF-GAA-ndhJ_p5	AATGAGGATGCGACATCAGG	GGATAGGATGGCCTTTACGT	50.0	50.0	57.7	56.8	864-873 bp	1.0	68.5