Hotspot

ycf1-trnN-GUU

IRa · 121460-121790 · 331 bp

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Pi 0.0323
MarkerSeek score 63.70
Primer available yes
Label ycf1-trnN-GUU

Feature Metadata

label_name
ycf1-trnN-GUU
feature_type
igs
region
IRa
length_bp
331
variable_sites
15
pi
0.0323
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-trnN-GUU_p1 CCTTCTTCCATAACCCGAGC AAGCGAGCCCTTTCATTCAA 55.0 45.0 58.0 58.1 607-614 bp 1.0 84.4
ycf1-trnN-GUU_p2 CCTTCTTCCATAACCCGAGC ATATAGTACACCCGGCCGAA 55.0 50.0 58.0 58.0 918-945 bp 1.0 84.2
ycf1-trnN-GUU_p3 CCTTCTTCCATAACCCGAGC AACTCGGGTAATGGAATGGC 55.0 50.0 58.0 57.9 973-1000 bp 1.0 83.5
ycf1-trnN-GUU_p4 CCTTCTTCCATAACCCGAGC GCCTTTCCCTCTGGGTAATG 55.0 55.0 58.0 57.9 1020-1047 bp 1.0 83.3
ycf1-trnN-GUU_p5 GCTAACGTGGAGCTTGGTAA ATATAGTACACCCGGCCGAA 50.0 50.0 57.9 58.0 1209-1236 bp 1.0 82.8