Hotspot

trnN-GUU-rpl32

SSC · 105331-106271 · 941 bp

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Pi 0.0258
MarkerSeek score 67.40
Primer available yes
Label trnN-GUU-rpl32

Feature Metadata

label_name
trnN-GUU-rpl32
feature_type
igs
region
SSC
length_bp
941
variable_sites
34
pi
0.0258
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rpl32_p1 AGTCAGTTAACAGCCAACCG ACCGGACAGTCAAAAAGTTT 50.0 40.0 58.1 55.7 1042-1119 bp 0.666667 53.2
trnN-GUU-rpl32_p2 ACGACCAGTCAGTTAACAGC ACCGGACAGTCAAAAAGTTT 50.0 40.0 57.8 55.7 1048-1125 bp 0.666667 53.0
trnN-GUU-rpl32_p3 AGGATTCGAACCTACGACCA ACCGGACAGTCAAAAAGTTT 50.0 40.0 58.2 55.7 1061-1138 bp 0.666667 52.9
trnN-GUU-rpl32_p4 GGATTCGAACCTACGACCAG ACCGGACAGTCAAAAAGTTT 55.0 40.0 57.8 55.7 1060-1137 bp 0.666667 52.5
trnN-GUU-rpl32_p5 ACCACTGAGCTACTGAGGAA ACCGGACAGTCAAAAAGTTT 50.0 40.0 57.7 55.7 1018-1095 bp 0.666667 51.7