Hotspot

rpl36-infA

LSC · 76103-76196 · 94 bp

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Pi 0.043
MarkerSeek score 70.20
Primer available yes
Label rpl36-infA

Feature Metadata

label_name
rpl36-infA
feature_type
igs
region
LSC
length_bp
94
variable_sites
6
pi
0.043
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-infA_p1 CCTACGAATCAGGCGACATT TGATTCAACCAGGGGACGTA 50.0 50.0 57.8 58.4 208-209 bp 1.0 78.1
rpl36-infA_p2 CCTACGAATCAGGCGACATT TCCGTCGCAGTTTTATACGT 50.0 45.0 57.8 57.4 269-270 bp 1.0 75.2
rpl36-infA_p3 CCTACGAATCAGGCGACATT TATGATTCAACCAGGGGACG 50.0 50.0 57.8 57.0 210-211 bp 1.0 72.9
rpl36-infA_p4 CCTACGAATCAGGCGACATT GATTCAACCAGGGGACGTAT 50.0 50.0 57.8 57.0 207-208 bp 1.0 72.9
rpl36-infA_p5 CCTACGAATCAGGCGACATT ATGATTCAACCAGGGGACGT 50.0 50.0 57.8 59.0 209-210 bp 1.0 72.8