Hotspot

psaJ-rpl33

LSC · 63066-63566 · 501 bp

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Pi 0.0319
MarkerSeek score 71.90
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
501
variable_sites
24
pi
0.0319
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 GATGCCCTGTCATTTCCCTT AGAAATGCCCGTCGATTCTT 50.0 45.0 57.9 57.6 648-690 bp 1.0 79.0
psaJ-rpl33_p2 TGGTTTGGATCTTTAGCGGG AGAAATGCCCGTCGATTCTT 50.0 45.0 57.9 57.6 699-741 bp 1.0 78.9
psaJ-rpl33_p3 GGTTTGGATCTTTAGCGGGT AGAAATGCCCGTCGATTCTT 50.0 45.0 57.9 57.6 698-740 bp 1.0 78.9
psaJ-rpl33_p4 TGCCCTGTCATTTCCCTTTT AGAAATGCCCGTCGATTCTT 45.0 45.0 57.6 57.6 646-688 bp 1.0 77.0
psaJ-rpl33_p5 TCCAGATGCCCTGTCATTTC AGAAATGCCCGTCGATTCTT 50.0 45.0 57.6 57.6 652-694 bp 1.0 76.7