Hotspot

accD-psaI

LSC · 56018-56218 · 201 bp

Back to Ophrys

Pi 0.0232
MarkerSeek score 67.30
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
201
variable_sites
7
pi
0.0232
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AGGGCTTATTCGACCCAATC AAGAAGCCATTGCAATTGCC 50.0 45.0 57.4 57.9 375-638 bp 1.0 75.1
accD-psaI_p2 GACCCAATCGTACCACGTAA AAGAAGCCATTGCAATTGCC 50.0 45.0 57.3 57.9 364-627 bp 1.0 75.0
accD-psaI_p3 AGGGCTTATTCGACCCAATC CCATTGCAATTGCCGGAAAA 50.0 45.0 57.4 58.2 369-632 bp 1.0 74.4
accD-psaI_p4 GACCCAATCGTACCACGTAA CCATTGCAATTGCCGGAAAA 50.0 45.0 57.3 58.2 358-621 bp 1.0 74.3
accD-psaI_p5 GCTACATGGTTTCCTTCCCT AAGAAGCCATTGCAATTGCC 50.0 45.0 57.2 57.9 311-574 bp 1.0 73.9