Hotspot

trnQ-UUG-psbK

LSC · 6302-6638 · 337 bp

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Pi 0.0247
MarkerSeek score 57.40
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
337
variable_sites
12
pi
0.0247
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 TAGGACCAAAACCCATTGCC AGACAGATACAGATTAAACTAAAGA 50.0 28.0 58.1 53.5 413-415 bp 1.0 42.5
trnQ-UUG-psbK_p2 TAGGACCAAAACCCATTGCC AAGACAGATACAGATTAAACTAAAGA 50.0 26.9 58.1 54.2 414-416 bp 1.0 42.5
trnQ-UUG-psbK_p3 CAAAACCCATTGCCTTACCG AGACAGATACAGATTAAACTAAAGA 50.0 28.0 57.6 53.5 407-409 bp 1.0 42.5
trnQ-UUG-psbK_p4 GAAGGATTCGAACCTCCGAG AGACAGATACAGATTAAACTAAAGA 55.0 28.0 57.8 53.5 436-438 bp 1.0 42.4
trnQ-UUG-psbK_p5 TGGAAATAGTCTGGGACGGA AGACAGATACAGATTAAACTAAAGA 50.0 28.0 57.8 53.5 454-456 bp 1.0 42.3