Hotspot

ycf1

SSC · 123524-129130 · 5607 bp

Back to Ophiorrhiza

Pi 0.0104

MarkerSeek score 50.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5607
variable_sites: 87
pi: 0.0104
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGAGAATTCTTAGTACCAATCTT	TGGGGGAATACTCCTATCAATCA	30.4	43.5	52.9	58.2	185-191 bp	0.666667	30.9
ycf1_p2	TTGAGAATTCTTAGTACCAATCT	TGGGGGAATACTCCTATCAATCA	30.4	43.5	52.9	58.2	186-192 bp	0.666667	30.9
ycf1_p3	TGAGAATTCTTAGTACCAATCTT	CAACCCTTTTGATATATGGGGGA	30.4	43.5	52.9	57.9	201-207 bp	0.666667	30.6
ycf1_p4	TTGAGAATTCTTAGTACCAATCT	CAACCCTTTTGATATATGGGGGA	30.4	43.5	52.9	57.9	202-208 bp	0.666667	30.6
ycf1_p5	TGAGAATTCTTAGTACCAATCTT	ACAACCCTTTTGATATATGGGGG	30.4	43.5	52.9	58.2	202-208 bp	0.666667	30.6