Hotspot

trnN-GUU-trnR-ACG

IRa · 129530-129951 · 422 bp

Back to Ophiorrhiza

Pi 0.00948

MarkerSeek score 66.10

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 422
variable_sites: 6
pi: 0.00948
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGTCGTAGGTTCGAATCCT	AACAGATCCAGTGGAGACGA	50.0	50.0	58.2	58.1	499-551 bp	1.0	83.0
trnN-GUU-trnR-ACG_p2	CGATTGGTCGTAGGTTCGAA	AACAGATCCAGTGGAGACGA	50.0	50.0	57.7	58.1	503-555 bp	1.0	82.2
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	463-515 bp	1.0	82.0
trnN-GUU-trnR-ACG_p4	TTCCTCAGTAGCTCAGTGGT	AACAGATCCAGTGGAGACGA	50.0	50.0	57.7	58.1	542-594 bp	1.0	81.7
trnN-GUU-trnR-ACG_p5	GGTTCGAATCCTACTTGGGG	AACAGATCCAGTGGAGACGA	55.0	50.0	57.7	58.1	491-543 bp	1.0	81.7