Hotspot

rpl32-trnL-UAG

SSC · 113056-113912 · 857 bp

Back to Ophiorrhiza

Pi 0.017

MarkerSeek score 60.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 857
variable_sites: 21
pi: 0.017
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTTGTACAGCAAACAAATAAGT	AGCAGCGTGTCTACCAATTT	27.3	45.0	52.8	57.8	889-913 bp	1.0	44.2
rpl32-trnL-UAG_p2	TTTGTACAGCAAACAAATAAGT	CCTAAGAGCAGCGTGTCTAC	27.3	55.0	52.8	57.8	895-919 bp	1.0	44.2
rpl32-trnL-UAG_p3	TTTGTACAGCAAACAAATAAGT	CTAAGAGCAGCGTGTCTACC	27.3	55.0	52.8	57.8	894-918 bp	1.0	44.2
rpl32-trnL-UAG_p4	TTTGTACAGCAAACAAATAAGT	GAGCAGCGTGTCTACCAATT	27.3	50.0	52.8	58.3	890-914 bp	1.0	44.2
rpl32-trnL-UAG_p5	TTTGTACAGCAAACAAATAAGT	TCCTAAGAGCAGCGTGTCTA	27.3	50.0	52.8	57.9	896-920 bp	1.0	44.1