Hotspot

trnR-ACG-trnN-GUU

IRb · 108258-108679 · 422 bp

Back to Ophiorrhiza

Pi 0.00948

MarkerSeek score 66.10

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 422
variable_sites: 6
pi: 0.00948
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	AACAGATCCAGTGGAGACGA	TGGTCGTAGGTTCGAATCCT	50.0	50.0	58.1	58.2	499-551 bp	1.0	83.0
trnR-ACG-trnN-GUU_p2	AACAGATCCAGTGGAGACGA	CGATTGGTCGTAGGTTCGAA	50.0	50.0	58.1	57.7	503-555 bp	1.0	82.2
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	463-515 bp	1.0	82.0
trnR-ACG-trnN-GUU_p4	AACAGATCCAGTGGAGACGA	TTCCTCAGTAGCTCAGTGGT	50.0	50.0	58.1	57.7	542-594 bp	1.0	81.7
trnR-ACG-trnN-GUU_p5	AACAGATCCAGTGGAGACGA	GGTTCGAATCCTACTTGGGG	50.0	55.0	58.1	57.7	491-543 bp	1.0	81.7