Hotspot

accD-psaI

LSC · 58631-59417 · 787 bp

Back to Ophiorrhiza

Pi 0.0129

MarkerSeek score 66.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 787
variable_sites: 14
pi: 0.0129
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CGAGAAGGGGTTATTCGACC	CCATTGCAATTGCCGGAAAT	55.0	45.0	57.8	58.0	954-972 bp	1.0	83.2
accD-psaI_p2	CGAGAAGGGGTTATTCGACC	AAGAAGCCATTGCAATTGCC	55.0	45.0	57.8	57.9	960-978 bp	1.0	82.6
accD-psaI_p3	TTCCACGCTTTCCTTCCTTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.6	58.0	884-902 bp	1.0	82.2
accD-psaI_p4	GTTCCACGCTTTCCTTCCTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.4	58.0	885-903 bp	1.0	81.9
accD-psaI_p5	CCACGCTTTCCTTCCTTTGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.4	58.0	882-900 bp	1.0	81.9