Hotspot

ndhC-trnV-UAC

LSC · 50807-51242 · 436 bp

Back to Ophiorrhiza

Pi 0.0292

MarkerSeek score 62.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 436
variable_sites: 17
pi: 0.0292
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCAAAGTCGAATCGTGACCC	TGCGCCAATGTTTTTCAAGG	50.0	45.0	57.9	58.1	1651-1661 bp	1.0	80.1
ndhC-trnV-UAC_p2	CAAAGTCGAATCGTGACCCT	TGCGCCAATGTTTTTCAAGG	50.0	45.0	57.9	58.1	1650-1660 bp	1.0	80.1
ndhC-trnV-UAC_p3	TCAAAGTCGAATCGTGACCC	GACAAAGGGTTCGGTTCGAT	50.0	50.0	57.9	58.2	1542-1552 bp	1.0	79.8
ndhC-trnV-UAC_p4	TCAAAGTCGAATCGTGACCC	AGGGCTATAGCTCAGTTGGT	50.0	50.0	57.9	57.8	1690-1700 bp	1.0	79.7
ndhC-trnV-UAC_p5	CAAAGTCGAATCGTGACCCT	AGGGCTATAGCTCAGTTGGT	50.0	50.0	57.9	57.8	1689-1699 bp	1.0	79.6