Pi
0.0091
MarkerSeek score
65.50
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 814-836 bp | 1.0 | 82.4 |
| trnT-UGU-trnL-UAA_p2 | GACCGTCGCATTACAAATGC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.2 | 58.0 | 832-854 bp | 1.0 | 81.9 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 822-844 bp | 1.0 | 81.8 |
| trnT-UGU-trnL-UAA_p4 | GACCGTCGCATTACAAATGC | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.2 | 58.1 | 840-862 bp | 1.0 | 81.3 |
| trnT-UGU-trnL-UAA_p5 | GCGATGCTCTAACCTCTGAG | GGGTTTCCATACCAAGGCTC | 55.0 | 55.0 | 57.9 | 58.2 | 858-880 bp | 1.0 | 80.4 |