Hotspot

trnY-GUA-trnE-UUC

LSC · 30874-30932 · 59 bp

Back to Ophiorrhiza

Pi 0.0565

MarkerSeek score 78.90

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 5
pi: 0.0565
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	TGGATTTGAACCAGCGTAGG	TATGATGGCGGTTGGACAAG	50.0	50.0	57.9	58.0	229 bp	1.0	80.6
trnY-GUA-trnE-UUC_p2	ATTTGAACCAGCGTAGGCAT	TATGATGGCGGTTGGACAAG	45.0	50.0	57.9	58.0	226 bp	1.0	80.4
trnY-GUA-trnE-UUC_p3	TGGATTTGAACCAGCGTAGG	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.9	58.3	178 bp	1.0	79.3
trnY-GUA-trnE-UUC_p4	GATTGACGAATTGTTGGGCC	TATGATGGCGGTTGGACAAG	50.0	50.0	57.7	58.0	253 bp	1.0	79.1
trnY-GUA-trnE-UUC_p5	TGGATTTGAACCAGCGTAGG	GTATGATGGCGGTTGGACAA	50.0	50.0	57.9	58.3	230 bp	1.0	78.8