Hotspot

atpH-atpI

LSC · 13564-14590 · 1027 bp

Back to Ophiorrhiza

Pi 0.0052

MarkerSeek score 63.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1027
variable_sites: 8
pi: 0.0052
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1109-1115 bp	1.0	76.8
atpH-atpI_p2	TACCCTCTACAGCTTGACCC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.1	58.5	1192-1198 bp	1.0	76.0
atpH-atpI_p3	GCGATACCCTCTACAGCTTG	TTTTTGCAACTTTAGCCGCG	55.0	45.0	57.9	58.5	1196-1202 bp	1.0	75.9
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	1110-1116 bp	1.0	75.7
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	1124-1130 bp	1.0	75.4