Hotspot

trnH-GUG-psbA

LSC · 79-308 · 230 bp

Back to Ophiorrhiza

Pi 0.0667
MarkerSeek score 76.10
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
230
variable_sites
23
pi
0.0667
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 300-310 bp 1.0 84.4
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 314-324 bp 1.0 84.1
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 313-323 bp 1.0 84.1
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 315-325 bp 1.0 84.1
trnH-GUG-psbA_p5 GCCTTGATCCACTTGGCTAC TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.6 57.9 305-315 bp 1.0 80.4