Hotspot

rpl16

LSC · 82678-84115 · 1438 bp

Back to Ophiopogon

Pi 0.00718

MarkerSeek score 53.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1438
variable_sites: 22
pi: 0.00718
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CCTCAATACCCCTTTTGTTT	CTTTTTCCGTTAAATCAAACAA	40.0	27.3	53.3	52.1	1564-1594 bp	1.0	42.3
rpl16_p2	CCTCAATACCCCTTTTGTTT	CTTTTTCCGTTAAATCAAACAAA	40.0	26.1	53.3	53.0	1564-1594 bp	1.0	42.3
rpl16_p3	CCTCAATACCCCTTTTGTTT	CTTTTTCCGTTAAATCAAACAAAA	40.0	25.0	53.3	53.8	1564-1594 bp	1.0	42.3
rpl16_p4	CCTCAATACCCCTTTTGTTTATCT	CTTTTTCCGTTAAATCAAACAA	37.5	27.3	56.6	52.1	1564-1594 bp	1.0	42.3
rpl16_p5	CCTCAATACCCCTTTTGTTTATCT	CTTTTTCCGTTAAATCAAACAAA	37.5	26.1	56.6	53.0	1564-1594 bp	1.0	42.3