Hotspot

infA

LSC · 81247-81483 · 237 bp

Back to Ophiopogon

Pi 0.0295

MarkerSeek score 67.10

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 237
variable_sites: 15
pi: 0.0295
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	AGCTTCGAGTTGTATCTCTCAC	AGTTGATGATATTTCAAGGGGT	45.5	36.4	57.6	55.1	283-315 bp	1.0	47.9
infA_p2	AGCTTCGAGTTGTATCTCTCAC	TGATGATATTTCAAGGGGTTACC	45.5	39.1	57.6	56.0	280-312 bp	1.0	47.9
infA_p3	AGCTTCGAGTTGTATCTCTCAC	GTTGATGATATTTCAAGGGGTTACC	45.5	40.0	57.6	58.0	282-314 bp	1.0	47.9
infA_p4	GCTTCGAGTTGTATCTCTCACC	AGTTGATGATATTTCAAGGGGT	50.0	36.4	58.6	55.1	282-314 bp	1.0	47.9
infA_p5	AGCTTCGAGTTGTATCTCTCAC	AGTTGATGATATTTCAAGGGGTT	45.5	34.8	57.6	55.9	283-315 bp	1.0	47.9